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Why Is Expression Ability the Top Criterion for Precision Fermentation Microbial Hosts?
Selecting microbial hosts for precision fermentation prioritizes the ability to express target ingredients via genetic engineering, alongside metabolic efficiency, safety, and scalability for high-yield production.
Question
Which of these is a key factor in selecting a microbial host for precision fermentation?
A. Ability to grow without feedstock
B. Resistance to carbon dioxide
C. Ability to express the desired target ingredient
D. Ability to survive extreme heat
Answer
C. Ability to express the desired target ingredient
Explanation
In precision fermentation, selecting a microbial host like yeast (Saccharomyces cerevisiae) or bacteria (E. coli) hinges primarily on its capacity for efficient expression of heterologous genes encoding the target ingredient, such as proteins like insulin or collagen, through well-established genetic tools like CRISPR for pathway integration and high-yield production. Key considerations include the host’s native biosynthetic compatibility, metabolic flux redirection to maximize titers, GRAS (Generally Recognized as Safe) status for food applications, and genetic tractability to minimize by-products and ensure scalability, distinguishing viable hosts from those unable to deliver the precise molecule at commercial volumes.